Cardiovascular disease is the main cause of mortality and morbidity in developed countries causing atherosclerosis plaques and thrombosis. Endothelial cell apoptosis is one of the main factors in vascular thrombosis. Lipids oxidized by endothelial cells lead to an increase thickness of the arterial wall. Iron is also known as an atherogenic element that causes atherosclerosis. As there is still doubt about the antioxidative role of vitamin E in atherosclerosis formation. In this study we evaluated the effect of iron and vitamin E on endothelial cell apoptosis of the carotid arteries inhypercholesteremicmale rabbits.
Thirty white male rabbits were randomly divided into 5 groups, and fed the following diet for 6 weeks: Group 1: control, Group 2: cholesterol (1%), group 3: cholesterol (1%) + vitamin E (50 mg/kg), group 4: cholesterol (1%) + Iron (50 mg/kg) and group 5: cholesterol (1%) + vitamin E (50 mg/kg) + Iron (50 mg/kg). Serum cholesterol, TG, HDL, and LDL levels were assessed after 6 weeks. Finally, the animals were sacrificed with ketamine and carotid arteries removed. The samples were fixed in 10% formalin and TUNEL staining was usedafter the tissue processing. Cell count were carried out under a light microscope.
Vitamin E decreased Serum cholesterol and apoptotic endothelial cells in hypercholesteremia+ vitamin E diet (P< 0/05). However, they increased significantly in the interference groups in comparison to the control group (P< 0/05).
According to our findings, vitamin E showed to have a beneficial effect on preventing cardiovascular diseases and atherosclerosis.