ARYA Atherosclerosis Journal

Serum levels of C1q/TNF-related protein-1 (CTRP1) and CTRP3 in patients with coronary artery disease

Document Type : Original Article

Authors

Gholamreza Namazi 1, 2
Maryam Mehranian 2
Alireza Mafi 2
Razieh Salami 2
Fariba Raygan 3

1 Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran

2 Department of Clinical Biochemistry, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran

3 Department of Cardiology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran

https://doi.org/10.48305/arya.2025.43204.3006
Abstract
BACKGROUND: Research suggests that C1q/TNF-related proteins (CTRPs) play a role in inflammation and may impact coronary artery disease (CAD). This study aimed to measure their levels in CAD patients and assess their correlation with disease severity.
METHODS: This study involved 88 individuals (50 men and 38 women) who underwent coronary angiography. Among them, 47 patients had significant coronary obstruction and were selected as the case group, and 41 individuals without significant obstruction were considered as the control group. Serum levels of CTRP1 and CTRP3 were quantified using the ELISA method, whereas other parameters were assessed by standard laboratory techniques. Multiple logistic regression analysis was used to examine the association between CTRP1 and CTRP3 levels with CAD.
RESULTS: The mean age of CAD patients was 62.1 ± 11.5 years. Thirty-one patients were male and sixteen were female. The control group comprised nineteen men and twenty-two women, with a mean age of 62.3 ± 12.7 years. 
The concentrations of CTRP1 and CTRP3 proteins were significantly higher in individuals with CAD relative to controls. Except for serum HDL levels, which were lower in the CAD group than in the control group, there were no significant differences in the demographic or clinical features between the studied groups. A direct and significant association was discovered between the amounts of assessed adipokines and the severity of CAD. 
CONCLUSION: A greater concentration of both CTRP1 and CTRP3 was strongly associated with the presence and severity of CAD, indicating that these adipocytokines may serve as markers for CAD.

Keywords

C1q-TNF-related Protein-1
CTRP1
C1q-TNF-Related Protein-3
CTRP3, Coronary Artery Disease
1. Zhang Y, Liu C, Liu J, Guo R, Yan Z, Liu W, et al. Implications of C1q/TNF-related protein superfamily in patients with coronary artery disease. Sci Rep. 2020 Jan 21;10(1):878. https://doi.org/10.1038/s41598-020-57877-z
2. Liu Y, Wei C, Ding Z, Xing E, Zhao Z, Shi F, Tian Y, Zhang Y, Fan W, Sun L. Role of serum C1q/TNF-related protein family levels in patients with acute coronary syndrome. Front Cardiovasc Med. 2022 Aug 19;9:967918. https://doi.org/10.3389/fcvm.2022.967918
3. Xin Y, Lyu X, Wang C, Fu Y, Zhang S, Tian C, et al. Elevated circulating levels of CTRP1, a novel adipokine, in diabetic patients. Endocr J. 2014;61(9):841-7. https://doi.org/10.1507/endocrj.ej14-0016
4. Yang Y, Liu S, Zhang RY, Luo H, Chen L, He WF, et al. Association Between C1q/TNF-Related Protein-1 Levels in Human Plasma and Epicardial Adipose Tissues and Congestive Heart Failure. Cell Physiol Biochem. 2017;42(5):2130-43. https://doi.org/10.1159/000479915
5. A novel adipokine CTRP1 stimulates aldosterone production. FASEB J. 2008 May;22(5):1502-11. https://doi.org/10.1096/fj.07-9412com
6. Wölfing B, Buechler C, Weigert J, Neumeier M, Aslanidis C, Schöelmerich J, et al. Effects of the new C1q/TNF-related protein (CTRP-3) “cartonectin” on the adipocytic secretion of adipokines. Obesity (Silver Spring). 2008 Jul;16(7):1481-6. https://doi.org/10.1038/oby.2008.206
7. Maeda T, Wakisaka S. CTRP3/cartducin is induced by transforming growth factor-beta1 and promotes vascular smooth muscle cell proliferation. Cell Biol Int. 2010 Jan 29;34(3):261-6. https://doi.org/10.1042/cbi20090043
8. Hou M, Liu J, Liu F, Liu K, Yu B. C1q tumor necrosis factor-related protein-3 protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis through the phosphoinositide 3-kinase/Akt pathway. Int J Mol Med. 2014 Jan;33(1):97-104. https://doi.org/10.3892/ijmm.2013.1550
9. Wang S, Ling Y, Liang W, Shen L. Association of serum C1q/TNF-related protein-3 (CTRP-3) in patients with coronary artery disease. BMC Cardiovasc Disord. 2017 Jul 28;17(1):210. https://doi.org/10.1186/s12872-017-0646-7
10. Fadaei R, Moradi N, Baratchian M, Aghajani H, Malek M, Fazaeli AA, et al. Association of C1q/TNF-Related Protein-3 (CTRP3) and CTRP13 Serum Levels with Coronary Artery Disease in Subjects with and without Type 2 Diabetes Mellitus. PLoS One. 2016 Dec 29;11(12):e0168773. https://doi.org/10.1371/journal.pone.0168773
11. Choi KM, Hwang SY, Hong HC, Choi HY, Yoo HJ, Youn BS, et al. Implications of C1q/TNF-related protein-3 (CTRP-3) and progranulin in patients with acute coronary syndrome and stable angina pectoris. Cardiovasc Diabetol. 2014 Jan 13;13:14. https://doi.org/10.1186/1475-2840-13-14
12. Schmid A, Pankuweit S, Vlacil AK, Koch S, Berge B, Gajawada P, et al. Decreased circulating CTRP3 levels in acute and chronic cardiovascular patients. J Mol Med (Berl). 2024 May;102(5):667-77. https://doi.org/10.1007/s00109-024-02426-8
13. Hammami R, Jdidi J, Mroua F, Kallel R, Hentati M, Abid L, et al. Accuracy of the TIMI and GRACE scores in predicting coronary disease in patients with non-ST-elevation acute coronary syndrome. Rev Port Cardiol (Engl Ed). 2018 Jan;37(1):41-9. https://doi.org/10.1016/j.repc.2017.05.012
14. Gao L, Lu D, Xia G, Zhang H. The relationship between arterial stiffness index and coronary heart disease and its severity. BMC Cardiovasc Disord. 2021 Nov 8;21(1):527. https://doi.org/10.1186/s12872-021-02350-6
15. Khandelwal G, Jain A, Rathore M. Prediction of Angiographic Extent of Coronary Artery Disease on the Basis of Clinical Risk Scores in Patients of Unstable Angina. J Clin Diagn Res. 2015 Nov;9(11):OC13-6. https://doi.org/10.7860/jcdr/2015/15069.6778
16. Namazi G, Asa P, Movahedian A, Sarrafzadegan N, Sadeghi M, Pourfarzam M. Investigation of membrane fatty acid profiles in erythrocytes of patients with stable coronary artery disease. J Clin Lipidol. 2016 Jul-Aug;10(4):930-6. https://doi.org/10.1016/j.jacl.2016.03.011
17. Yuasa D, Ohashi K, Shibata R, Takeshita K, Kikuchi R, Takahashi R, et al. Association of circulating C1q/TNF-related protein 1 levels with coronary artery disease in men. PLoS One. 2014 Jun 19;9(6):e99846. https://doi.org/10.1371/journal.pone.0099846
18. Tang JN, Shen DL, Liu CL, Wang XF, Zhang L, Xuan XX, et al. Plasma levels of C1q/TNF-related protein 1 and interleukin 6 in patients with acute coronary syndrome or stable angina pectoris. Am J Med Sci. 2015 Feb;349(2):130-6. https://doi.org/10.1097/maj.0000000000000378
19. Wang H, Wang R, Du D, Li F, Li Y. Serum levels of C1q/TNF-related protein-1 (CTRP-1) are closely associated with coronary artery disease. BMC Cardiovasc Disord. 2016 May 11;16:92. https://doi.org/10.1186/s12872-016-0266-7
20. Lu L, Zhang RY, Wang XQ, Liu ZH, Shen Y, Ding FH, et al. C1q/TNF-related protein-1: an adipokine marking and promoting atherosclerosis. Eur Heart J. 2016 Jun 7;37(22):1762-71. https://doi.org/10.1093/eurheartj/ehv649
21. Shen L, Wang S, Ling Y, Liang W. Association of C1q/TNF-related protein-1 (CTRP1) serum levels with coronary artery disease. J Int Med Res. 2019 Jun;47(6):2571-9. https://doi.org/10.1177/0300060519847372
22. Zhang H, Zhang-Sun ZY, Xue CX, Li XY, Ren J, Jiang YT, et al. CTRP family in diseases associated with inflammation and metabolism: molecular mechanisms and clinical implication. Acta Pharmacol Sin. 2023 Apr;44(4):710-25. https://doi.org/10.1038/s41401-022-00991-7
23. Lasser G, Guchhait P, Ellsworth JL, Sheppard P, Lewis K, Bishop P, et al. C1qTNF-related protein-1 (CTRP-1): a vascular wall protein that inhibits collagen-induced platelet aggregation by blocking VWF binding to collagen. Blood. 2006 Jan 15;107(2):423-30. https://doi.org/10.1182/blood-2005-04-1425
24. Masoodian SM, Toolabi K, Omidifar A, Zabihi H, Rahimipour A, Shanaki M. Increased mRNA Expression of CTRP3 and CTRP9 in Adipose Tissue from Obese Women: Is it Linked to Obesity-Related Parameters and mRNA Expression of Inflammatory Cytokines? Rep Biochem Mol Biol. 2020 Apr;9(1):71-81. https://doi.org/10.29252/rbmb.9.1.71
25. Peng C, Wu H, Kim S, Dai X, Jiang X. Recent Advances in Transducers for Intravascular Ultrasound (IVUS) Imaging. Sensors (Basel). 2021 May 19;21(10):3540. https://doi.org/10.3390/s21103540
26. Marteslo JP, Makary MS, Khabiri H, Flanders V, Dowell JD. Intravascular Ultrasound for the Peripheral Vasculature-Current Applications and New Horizons. Ultrasound Med Biol. 2020 Feb;46(2):216-24. https://doi.org/10.1016/j.ultrasmedbio.2019.10.010
ARYA Atherosclerosis Journal
Volume 21, Issue 5
May and June 2025
Pages 34-41

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