ARYA Atherosclerosis Journal

Role of A Single Nucleotide Polymorphism in Thrombospondine 4 Gene in Premature Myocardial Infarction among Population of Southern Iran

Document Type : Original Article(s)

Authors

Nazanin Farahbakhsh 1
Zahra Hooshanginezhad 2
Shiva Saleh 3
Fariba Alaei 4
Fatemeh Azizi 5
Mohammad Shojaie 6

1 Pediatric Pulmonology Department, Mofid Children’s Hospital, Shahid Beheshti University of medical Sciences, Tehran, Iran

2 Department of Cardiology, Jahrom University of Medical Sciences, Jahrom, Fars, Iran

3 Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Fars, Iran

4 Department of Pediatric Cardiology, Mofid Children’s Hospital, Shahid Beheshti University of medical Sciences, Tehran, Iran

5 Department of Cardiology, Shiraz University of Medical Sciences, Shiraz, Fars, Iran

6 Cardiology Department, Jahrom University of Medical Sciences. Jahrom, Fars, Iran

https://doi.org/10.48305/arya.2023.41105.2846
Abstract
Background: Coronary Artery Diseases (CAD) are the leading cause of Myocardial Infarction (MI). However, their underlying etiology can be found in the interplay between environmental and genetic factors. On the other hand, it has been shown that Extracellular Matrix (ECM) proteins, such as Thrombospondins (TSP), play a crucial regulatory role in vascular pathologies, including atherogenesis. TSPs are extracellular proteins responsible for intercellular and cell-ECM interactions and are involved in regulating functional responses. Recently, a missense mutation in the TSP-4 gene has been reported to potentially increase the risk of CADs. The present study aimed to investigate the role of rs1866389 Guanosine to Cytosine (G/C) Single Nucleotide Polymorphism (SNP) of the TSP-4 gene on the prevalence of premature MI in southern Iran.
Methods: The present case-control study included 100 patients with premature MI and 100 healthy individuals. The DNA extracted from the blood samples of the participants underwent Polymerase Chain Reaction (PCR) for the sequence of the TSP-4 gene. Afterward, the frequency of C (mutated) and G (normal) alleles of the TSP-4 gene was evaluated in the case and control groups. 
Results: According to our findings, there was no significant intergroup difference in gender, age, and smoking status. However, the case group was significantly higher in the prevalence of Diabetes mellitus (DM), Hyperlipidemia (HLP), and Hypertension (HTN) compared to the control group. Moreover, 22%, 49%, and 29% of the case group had CC, GC, and GG genotypes in the TSP-4 gene, respectively, while the prevalence of CC, GC, and GG genotypes were 10%, 44%, and 46% in the control group. Also, the prevalence of allele C was significantly higher in the case group (47%) compared to the control group (33%, P=0.043), showing its significant association with the increased risk of premature MI (OR = 1.80; 95% CI = 1.01-3.19). 
Conclusions: The rs1866389 G/C SNP of the TSP-4 gene significantly increased the risk of premature MI in the population of southern Iran. Thus, such mutated gene can be used as a target for gene therapy or a marker for early detection of individuals at high risk for CADs. 

Highlights

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Keywords

Coronary Artery Disease
Myocardial Infarction
Extracellular Matrix Protein
Thrombospondin Gene
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ARYA Atherosclerosis Journal
Volume 19, Issue 2
February 2023
Pages 23-29

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