Document Type : Original Article(s)


1 Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran

2 Department of Clinical Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

3 Seyyed-al Shohada University Hospital, Urmia University of Medical Sciences, Urmia, Iran


Background: The purpose of this study was to identify the angiotensin-converting enzyme (ACE) gene (I/D) variations in CAD patients and healthy controls in an Iranian population (West Azerbaijan province of Iran).
Methods: This cross-sectional study included 95 CAD patients and 203 healthy controls. ACE I/D polymorphisms were assessed using PCR, and their frequency was determined. 
Results: There were 298 people, 95 CAD patients, and 203 controls, with an average age of 50.96±3.45 and 51.14±10.20. We discovered that the frequency of the D allele was significantly higher in CAD patients than in controls (P = 0.0009). In contrast, the frequency of the I allele was significantly higher in controls than in CAD patients (P = 0.0009). The D allele carriers genotypes (DD + ID) were more frequent in the CAD patients than in the control group (P = 0.008). The ACE II genotype-state carriers were more common in the control group than in CAD patients (P = 0.008). However, in the case of the ACE ID genotype, no significant differences were not found in the tested groups (P = 0.47).

Conclusions: These findings suggest that individuals with the ACE DD genotype are predisposed to CAD, whereas individuals with the ACE II genotype state are protected.


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Azova M, Timizheva K, Ait Aissa A, Blagonravov M, Gigani O, Aghajanyan A, Tskhovrebova L. Gene Polymorphisms of the Renin-Angiotensin-Aldosterone System as Risk Factors for the Development of In-Stent Restenosis in Patients with Stable Coronary Artery Disease. Biomolecules. 2021; 11(5): 763.
Bagheri M, Abdi Rad I, Omrani MD, Nanbaksh F. Polymorphisms of the angiotensin converting enzyme gene in Iranian Azeri Turkish women with unexplained recurrent pregnancy loss. Hum Fertil (Camb). 2010; 13(2): 79-82.
Abdi RI, Bagheri M. Angiotensin-converting enzyme insertion/deletion gene polymorphism in general population of west Azarbaijan, Iran. Iran J Kidney Dis. 2011; 5(2): 86-92.
Mohebbi I, Rad IA, Bagheri M. Association of angiotensin-1-converting enzyme gene variations with silicosis predisposition. Inhal Toxicol. 2010; 22(13): 1110-5.
Zhang Y, Yang T, Zhou W, Huang Y. A meta-analysis on the association of genetic polymorphism of the angiotensin-converting enzyme and coronary artery disease in the chinese population. Rev Assoc Med Bras  .(1992)2019; 65(6): 923-9.
de Carvalho SS, Simões e Silva AC, Sabino Ade P, Evangelista FC, Gomes KB, Dusse LM, Rios DR. Influence of ACE I/D Polymorphism on Circulating Levels of Plasminogen Activator Inhibitor 1, D-Dimer, Ultrasensitive C-Reactive Protein and Transforming Growth Factor β1 in Patients Undergoing Hemodialysis. PLoS One. 2016; 11(3): e0150613.
Bukowska A, Spiller L, Wolke C, Lendeckel U, Weinert S, Hoffmann J, et al. Protective regulation of the ACE2/ACE gene expression by estrogen in human atrial tissue from elderly men. Exp Biol Med (Maywood). 2017; 242(14): 1412-23.
Amara A, Mrad M, Sayeh A, Lahideb D, Layouni S, Haggui A, et al. The Effect of ACE I/D Polymorphisms Alone and With Concomitant Risk Factors on Coronary Artery Disease. Clin Appl Thromb Hemost. 2018; 24(1): 157-163. 
Ghaffarzadeh A, Bagheri M, Khadem-Vatani K, Abdi Rad I. Association of MMP-1 (rs1799750)-1607 2G/2G and MMP-3 (rs3025058)-1612 6A/6A Genotypes With Coronary Artery Disease Risk Among Iranian Turks. J Cardiovasc Pharmacol. 2019; 74(5): 420-425. 
Miller SA, Dykes D, Polesky H. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988; 16(3): 1215. 
Borai IH, Hassan N, Shaker O, Ashour E, et al. Synergistic effect of ACE and AGT genes in coronary artery disease. Beni-Suef Uni J Basic Appl Sci. 2018; 7(1):111-7.
Paz Ocaranza M, Riquelme JA, García L, Jalil JE, Chiong M, Santos RAS, Lavandero S. Counter-regulatory renin-angiotensin system in cardiovascular disease. Nat Rev Cardiol. 2020; 17(2): 116-129. 
Niemiec P, Zak I, Wita K. Modification of the coronary artery disease risk associated with the presence of traditional risk factors by insertion/deletion polymorphism of the ACE gene. Genet Test. 2007; 11(4): 353-9.
Zintzaras E, Raman G, Kitsios G, Lau J. Angiotensin-converting enzyme insertion/deletion gene polymorphic variant as a marker of coronary artery disease: a meta-analysis. Arch Intern Med. 2008; 168(10): 1077-89.
Study of angiotensin-converting enzyme insertion/deletion polymorphism, enzyme activity and oxidized low density lipoprotein in Western Iranians with atherosclerosis: a case-control study. BMC Cardiovasc Disord. 2019; 19(1): 184.