Document Type : Original Article
Authors
- Muhamad Robiul Fuadi 1
- Jusak R Nugraha 2
- I Gde Rurus Suryawan 3
- Hartono Kahar 4
- Aryati Aryati 4
- Gwenny Ichsan Prabowo 5
- Budi Utomo 6
- Reny I'tishom 7
1 Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
2 Department of Clinical Pathology , Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
3 Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
4 Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
5 Department of Physiology and Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
6 Department of Public Health and Preventive Medicine Faculty of Medicine, Airlangga University, Surabaya
7 Department of Medical Biology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
Abstract
Background: Cardiovascular disease (CVD) is the most common cause of mortality globally. One of the CVD manifestations is acute coronary syndrome (ACS). There weren’t many studies about genetics as a potential risk factor for ACS in Indonesia. This study aims to analyze the effect of Methylenetetrahydrofolate Reductase (MTHFR) A1298C gene polymorphism on ACS incidence.
Method: It was a case-control design study. Subjects consisted of 90 cardiology and internal medicine clinic outpatients at the University of Airlangga (UNAIR) Hospital Surabaya, Indonesia. There were 60 patients in the case group with a history of ACS, while the control group consisted of 30 patients without cardiovascular complaints during their lifetime. MTHFR A12980C gene polymorphism examination was performed using the polymerase chain reaction-restriction fragment length polymorphism ( PCR RFLP) method at Tropical Disease Center UNAIR Laboratory.
Results: ACS group had AA, AC, and CC genotype patterns, respectively 29 (48.3%), 13 (21.7%), and 18 (30%) subjects. Genotype patterns of control group were AA:16 (53.3%), AC: 6 (20.0%), and CC: 8 (26.7%) subjects. C allele variant was found in 41% of ACS group, and 37% in control group. Odds ratio for incidence of ACS in subjects with genotypes AC, CC, and C allele were 1.195 (95% CI 0.381-3.752), 1.241 (95% CI 0.442-3.486), and 1.222 (0.508-2.939). There wasn’t relationship between MTHFR A1298C gene polymorphism with incidence of ACS by Chi-square analysis (p > 0.05).
Conclusion: MTHFR A1298C gene polymorphism did not significantly affect ACS incidence.
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