Document Type : Original Article(s)

Authors

1 Department of Medical Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

2 Department of Cardiology, School of Medicine, Research Center for Prevention of Cardiovascular Endocrinology & Metabolism, Research Institute Hazrate Rasool General Hospital, Iran University of Medical Sciences, Tehran, Iran

3 Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

4 Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Research Center of Pediatric Infectious Diseases, Institute of Immunology & Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran

5 Infectious Disease Research Center, Birjand University of Medical Sciences, Birjand,Iran

6 Department of Bacteriology and Virology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran

7 Department of Medical Laboratory Sciences, Faculty of Para Medicine, Alborz University of Medical Sciences, Karaj, Iran

8 Product manager of IBRF, Iranian Blood Transfusion Organization, Tehran, Iran

9 Department of Medical Virology, Iran University of Medical Sciences, Tehran, Iran

Abstract

Background: Coronary artery disease (CAD) is a leading cause of death around the world. MiRNAs can be involved in the formation of atherosclerotic plaques, inflammation, cholesterol metabolism, and other mechanisms involved in CAD development. This study aimed to evaluate the expression level of miR-22, 30c, 145, and 519d and their possible association with inflammatory markers among CAD patients.Method: The expression level of miR-22, miR-30c, miR-145, miR-519d, IL-6, and TGF-B was determined in 46 CAD patients and 39 healthy controls using real-time polymerase chain reaction (PCR) assay.Result: The miRNA expression pattern of each group showed significantly different expression profiles. In the CAD patients group, miR-22, miR-30c, and miR-145 were down-regulated compared to the control group. On the opposite, miR-519d was up-regulated in CAD patients compared to the control group. Our results also showed that the expression levels of IL-6 and TGF-B were up-regulated among CAD patients compared to the control group. In addition, the expression of miR-145 and miR-519d had a significantly negative and positive correlation with TGF-B and IL-6 respectively.Conclusion: Our result showed that the change in expression levels of miR-22, miR-30c, miR-145, and miR-519d in PBMCs of CAD patients could be considered as potential biomarkers for CAD.

Keywords