TY - JOUR ID - 10733 TI - The effects of nanomicelle of curcumin on the matrix metalloproteinase (MMP-2, 9) activity and expression in patients with coronary artery disease (CAD): A randomized controlled clinical trial JO - ARYA Atherosclerosis Journal JA - ARYA LA - en SN - 1735-3955 AU - Mogharrabi, Marzieh AU - Rahimi, Hamid Reza AU - Hasanzadeh, Shima AU - Dastani, Mostafa AU - Kazemi-Oskuee, Reza AU - Akhlaghi, Saeed AU - Soukhtanloo, Mohammad AD - Associate Professor, Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AD - Assistant Professor, Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran AD - Associate Professor, Cardiovascular Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AD - Associate Professor, Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AD - Assistant Professor, Psychiatry and Behavioral Sciences Research Center AND Department of Psychiatry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AD - Associate Professor, Pharmacological Research Center of Medicinal Plants AND Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Y1 - 2020 PY - 2020 VL - 16 IS - 3 SP - 136 EP - 145 KW - Curcumin KW - Matrix Metalloproteinases KW - Coronary Artery Disease DO - 10.22122/arya.v16i3.1938 N2 - BACKGROUND: Coronary artery disease (CAD) is the most common type of cardiovascular disease. Increasing the expression and activity of matrix metalloproteinases (MMPs) facilitates vascular remodeling and cardiovascular complications. Curcumin (the active ingredient of turmeric) is a potent natural anti-inflammatory agent, with cardiovascular protective effects. The present study was a clinical trial for investigating the effects of curcumin on activity and gene expression of MMP-2 and MMP-9 in patients with CAD.METHODS: In this study, 70 patients with CAD (with 40%-50% stenosis) were randomly divided into two groups of curcumin (80 mg nanomicelle per day) and placebo. The intervention lasted 3 months. The activity levels of MMP-2 and MMP-9 in serum samples of patients were measured using gelatin zymography assay before and after the intervention. MMP-2 and MMP-9 gene expression in peripheral blood mononuclear cells (PBMCs) was also analyzed using real-time polymerase chain reaction (PCR). Statistical significance was set at P < 0.0500.RESULTS: After 3 months of medication, the expression of MMP-9 produced by PBMCs significantly decreased in the curcumin group (0.811 ± 0.25) in comparison with the placebo group (2.23 ± 0.94) (P < 0.0001). Furthermore, the zymographic analysis showed that the administration of curcumin significantly inhibited the activity levels of MMP-2 (12469.7 ± 5308.64 pixels) and MMP-9 (14007.2 ± 5371.67 pixels) in comparison with that in patients receiving placebo (MMP-2: 17613.8 ± 5250.68 pixels; MMP-9: 20010.1 ± 3259.37 pixels) (P < 0.0500).CONCLUSION: Our results show that curcumin can significantly reduce the expression and activity of MMP-2 and MMP-9. Because of the anti-inflammatory effects of curcumin, this compound can be considered as a new strategy for the prevention of cardiovascular events. UR - https://arya.mui.ac.ir/article_10733.html L1 - https://arya.mui.ac.ir/article_10733_3e37e8f896544e6dbc38c99ee475e71a.pdf ER -