TY - JOUR ID - 10593 TI - Transcriptional activity of tumor necrosis factor-alpha gene in peripheral blood mononuclear cells in patients with coronary slow flow JO - ARYA Atherosclerosis Journal JA - ARYA LA - en SN - 1735-3955 AU - Rasmi, Yousef AU - Bagheri, Morteza AU - Faramarz-Gaznagh, Sanaz AU - Nemati, Mohadeseh AU - Khadem-Ansari, Mohammad Hasan AU - Saboory, Ehsan AU - Seyed-Mohamadzad, Mir Hossein AU - Shirpoor, Alireza AD - Professor, Cellular and Molecular Research Center AND Department of Biochemistry, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran AD - Assistant Professor, Cellular and Molecular Research Center AND Student Research Committee, Urmia University of Medical Sciences, Urmia, Iran AD - Department of Biochemistry, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran AD - Professor, Department of Biochemistry, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran AD - Professor, Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran AD - Associate Professor, Department of Cardiology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran AD - Associate Professor, Department of Physiology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran Y1 - 2017 PY - 2017 VL - 13 IS - 4 SP - 196 EP - 201 KW - Inflammation KW - Tumor Necrosis Factor-alpha KW - Cytokines KW - Slow Flow Phenomenon KW - Coronary Angiography DO - N2 - BACKGROUND: Coronary slow flow (CSF), an angiographic phenomenon that is characterized by a delayed coronary blood flow in the absence of obstructive coronary artery stenosis, is known as a disorder of the coronary microcirculation. Inflammation has an important role in the vascular hemostasis and endothelial dysfunction especially regarding monocyte adhesion and infiltration. Pro-inflammatory cytokines released by inflammatory cells result in endothelial cell dysfunction and cardiovascular diseases. It has been demonstrated that tumor necrosis factor-alpha (TNF-α) mainly influences the vascular homeostasis and endothelial dysfunction. In the present enquiry the transcriptional activity of TNF-α gene in peripheral blood mononuclear cells (PBMCs) of patients with CSF was compared with healthy controls in order to further survey the role of TNF-α in pathophysiology of CSF. METHODS: The study was carried out on 30 patients with CSF and 30 matched healthy controls. To analysis gene expression of TNF-α, total mRNA was isolated from PBMCs. The quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) was used to compare the transcriptional activity of TNF-α gene between patients with CSF and controls. RESULTS: The mean ± standard error of mean of fold in CSF patients and controls were 0.20 ± 0.04 and 1.38 ± 0.27, respectively. The mRNA mean expressions of TNF-α (fold) were different in tested groups, which indicated a significant decrease in TNF-α in patients with CSF group (P = 0.0001). CONCLUSION: Expression of TNF-α was decreased in patients with CSF. Changes in TNF-α expression suggest a potential role for altered immune function in the pathophysiology of CSF.  UR - https://arya.mui.ac.ir/article_10593.html L1 - https://arya.mui.ac.ir/article_10593_0a020a7c5db88a54f797776b2077a998.pdf ER -