%0 Journal Article %T Adiponectin inhibits oxidized low density lipoprotein-induced increase in matrix metalloproteinase 9 expression in vascular smooth muscle cells %J ARYA Atherosclerosis Journal %I Cardiovascular research institute, Isfahan University of Medical Sciences %Z 1735-3955 %A Saneipour, Maryam %A Ghatreh-Samani, Keihan %A Heydarian, Esfandiar %A Farrokhi, Effat %A Abdian, Narges %D 2015 %\ 06/28/2015 %V 11 %N 3 %P 191-195 %! Adiponectin inhibits oxidized low density lipoprotein-induced increase in matrix metalloproteinase 9 expression in vascular smooth muscle cells %K Matrix Metalloproteinase 9 %K Adiponectin %K Oxidized Low Density Lipoprotein %R %X BACKGROUND: High expression of matrix metalloproteinase 9 (MMP9) during vascular injury and inflammation plays an important role in atherosclerotic plaque formation and rupture. In the process of atherosclerosis, oxidized low-density lipoprotein (oxLDL) upregulates MMP9 in human aortic vascular smooth muscle cells (HA/VSMCs). Adiponectin is an adipose tissue-derived hormone that has been shown to exert anti-atherogenic and anti-inflammatory effects. The aim of this study was to investigate the effect of adiponectin on MMP9 expression under pathogenic condition created by oxLDL in HA/VSMCs. METHODS: In this experimental study, HA/VSMC were stimulated with oxLDL alone and in the presence of adiponectin for 24 and 48 h. The expression of MMP9 gene was determined by real-time polymerase chain reaction method. The protein level of this gene was investigated by western blotting technique. RESULTS: An oxLDL increased MMP9 expression 2.16 ± 0.24- and 3.32 ± 0.25-fold after 24 and 48 h, respectively and adiponectin decreased oxLDL-induced MMP9 expression in a time-dependent manner. CONCLUSION: These results show that adiponectin changes extracellular matrix by reducing MMP9 mRNA and protein, therefore, may stabilize lesions and reduce atheroma rupture.  %U https://arya.mui.ac.ir/article_10472_dad76b5cacaaca31ae06c492e28fddd8.pdf