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The mechanism of vascular adhesion molecule-1 in an ischemia-reperfusion cardia model

Document Type : Original Article

Authors

1 Student Research Committee, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

2 Department of Physiology and Pharmacology, School of Medicine, Cardiovascular Research Center, Lorestan University of Medical Sciences, Khorramabad, Lorestan, Iran

3 Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4 Department of Nutritional Health Research Center, School of Health and Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran

5 Faculty of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6 Department of physiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

BACKGROUND: Vascular adhesion molecule-1 (VCAM-1) is involved in promoting inflammation within blood vessels, activating endothelial cells, and is a key factor in the progression of diabetic vasculopathy in rats with diabetes, contributing to the underlying pathophysiological processes. This study focused on the expression level of VCAM-1 in diabetic rats subjected to a six-week schedule of aerobic training and valerian supplements.
METHODS: Fifty male Wistar rats’ hearts were removed under deep anesthesia and were studied using Lutgendorf’s apparatus. They were divided into five groups (10 each): Healthy control (C), Diabetic control (DC), Diabetic with valerian (DV), Diabetic with exercise (DE), and Diabetic with valerian and exercise (DVE). Diabetes was induced in the animals by administering a shot of STZ (50 mg/kg) in their abdominal area. Following confirmation of diabetes in the animals, moderate exercise five days a week, combined with intraperitoneal administration of 200 mg/kg/day of valerian, was maintained for six weeks. Heart tissue was obtained from diabetic cardiac ischemia-reperfusion model (CI/RM) injury (n=40) and control rats (n=10).
RESULTS: VCAM-1 expression and histological parameters were not observed when comparing experimental and control groups. However, the exercise/valerian treatment (E + V) notably reduced the irregularity in cardiac tissue and increased the size of cardiomyocytes.
CONCLUSION: These findings suggest that E + V extract could diminish the levels of diabetic cardiac complications. Also, it had a dual effect: it corrected cardiac tissue abnormalities and increased the size of cardiomyocytes, enhancing the overall structure and function of the heart. More research is needed to understand non-pharmacological complementary treatments in this area.

Keywords

References
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ARYA Atherosclerosis Journal
Volume 21, Issue 4
May and June 2025
Pages 44-54
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